Separate transcription and splicing gene networks are linked and coordinated by the pRb-E2F pathway.

ABSTRACT: The pRb-E2F pathway is involved in mediating diverse cell fates, and oncogenic disruption of the pathway is regarded as a hallmark of cancer. Recent studies highlighted the pRb-E2F axis as a regulator of a large gene network which includes RNA splicing and transcription targets. Here, we have performed a deep genome-wide analysis of differentially expressed genes (DEGs) and alternatively spliced (AS) RNA targets which highlighted broadly non-overlapping networks of genes that are independently regulated by the pRb-E2F pathway. Individual pathway components, including E2F1, pRb, and PRMT5, either as single or combined knockouts, were found to influence DEG and AS networks but to different extents. An analysis of the E2F1 interactome revealed SRSF2 and HNRNPC as candidate proteins that were

SUBMITTER: Carr SM 

PROVIDER: S-EPMC12856215 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature