Macrophage immunometabolic reprogramming impairs tissue regeneration in type 2 diabetes zebrafish model.

ABSTRACT:

Introduction

Type 2 diabetes mellitus (T2D) is a metabolic disorder characterized by chronic hyperglycemia, insulin resistance, and meta-inflammation, which significantly compromise tissue regeneration. Although macrophage dysfunction is implicated in impaired wound healing in T2D, the immunometabolic mechanisms linking hyperglycemia to defective tissue repair remain incompletely understood.

Methods

Zebrafish larvae were exposed to hyperglycemic conditions (4% dextrose) to establish a T2D-like model. Survival, glycemic and biochemical parameters were assessed, followed by caudal fin amputation to evaluate regenerative capacity. Total (Mpeg1⁺) and pro-inflammatory (Mpeg1⁺/TNF⁺) macrophages were quantified in vivo using confocal microscopy. Add

SUBMITTER: Junior LW 

PROVIDER: S-EPMC12856266 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature