Targeted blood proteome profiling using NULISAseq identifies a high-performance biomarker panel for Aβ pathology quantification and staging.

ABSTRACT:

Introduction

While current blood-based biomarkers for Alzheimer's disease (AD) are effective for determining amyloid beta (Aβ) pathology positivity/negativity, they are insufficient for quantifying Aβ plaque deposition.

Methods

We profiled 325 plasma proteins in a Hong Kong Chinese cohort using the Nucleic Acid Linked Immuno‑Sandwich Assay (NULISAseq) platform. We analyzed the dysregulation trajectories of the blood proteome along Aβ pathology progression and used machine learning to develop a biomarker panel to quantify Aβ pathology.

Results

We identified 43 blood proteins correlated with Aβ plaque accumulation and selected 8 proteins to construct a model. This model was strongly correlated with amyloid positron emission tomography Centiloid values (r = 0.89), enabli

SUBMITTER: Zheng W 

PROVIDER: S-EPMC12856377 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature