Streptomyces anthophorae sp. nov. and Streptomyces nidicola sp. nov., novel actinobacteria isolated from a solitary bee.

ABSTRACT: Six novel actinobacterial strains (BH034^T, BH055, BH097, BH104, BH105 and BH106^T) were isolated from developing brood in nests of the solitary bee species Anthophora bomboides from Bodega Bay, California, USA. Phylogenetic analysis based on a five-gene multilocus sequence alignment and whole-genome data positions all six strains within the genus Streptomyces, with close relation to Streptomyces endophyticus YIM 65594^T and Streptomyces fractus MV32^T. Through genetic and chemotaxonomic analysis, five of the isolates (BH034^T, BH055, BH097, BH104 and BH105) were found to be a clade representing one species [>96% average nucleotide identity (ANI)], whereas BH106^T was a distinct species (<93% ANI with each of the other isolate genomes). Within this species (BH034^T-BH105), the genomes comprised on average 9.6 Mb (±0.4 Mb), encoded 8,640 (±349) predicted genes and had a G+C content of 70.9 (±0.07) mol%. The type strain, BH034^T, contained iso-C_16 : 0, anteiso-C_15 : 0 and iso-C_15 : 0 as major fatty acids and contained ll-diaminopimelic acid in the cell wall. The remaining strain, BH106^T, represents a distinct species; its genome comprised 9.4 Mb, encoded 8,426 predicted genes and had a G+C content of 70.7 mol%; the major fatty acids were anteiso-C_15 : 0, anteiso-C_17 : 0, iso-C_17 : 0 and iso-C_15 : 0, and the cell wall also contained ll-diaminopimelic acid. Functional genomic analysis revealed multiple secondary metabolite gene clusters in the bee-associated Streptomyces strains, several of which were found to be absent in closely related Streptomyces species. Based on genotypic, phenotypic and chemotaxonomic analyses, strains BH034-BH105 and BH106 represent two novel species within the genus Streptomyces, for which the names Streptomyces anthophorae sp. nov. (type strain BH034^T=NRRL B-65741^T=DSM 119658^T) and Streptomyces nidicola sp. nov. (type strain BH106^T=NRRL B-65742^T=DSM 119659^T) are proposed.

SUBMITTER: Christensen SM 

PROVIDER: S-EPMC12856498 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature