Planar cell polarity protein Vangl2 interacts with M-cadherin and stabilizes its cell surface expression in mouse C2C12 myoblasts.

ABSTRACT: Skeletal muscle regeneration depends on muscle stem cells (MuSCs), in which cadherin-mediated adhesion and planar cell polarity (PCP) signaling play critical roles. M-Cadherin is the major cadherin expressed in MuSCs; however, its functional link to PCP proteins remains unclear. In this study, we demonstrate that the PCP core component Vangl2 co-localizes with M-cadherin at the MuSC-myofiber boundary and directly interacts with it in C2C12 cells. Mutagenesis analyses revealed that the catenin-binding domain of M-cadherin and the C-terminal domain of Vangl2 are required for this interaction, which uniquely enables M-cadherin to form a ternary complex with Vangl2 and β-catenin. Knockdown of Vangl2 impaired myoblast fusion, reduced the expression of MyoD and Myomixer, and decreased the cell s

SUBMITTER: Nagaoka T 

PROVIDER: S-EPMC12856500 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature