Unraveling pyrrolizidine alkaloid-induced liver damage with an integrative spatial lipidomics framework.

ABSTRACT: Pyrrolizidine alkaloids (PAs), a class of secondary metabolites widely distributed in plants and the accidental ingestion or improper use of foods and herbs containing PAs, can lead to irreversible liver damage. Considering that the toxic mechanism of PAs is closely associated with metabolism, the hepatotoxicity was analyzed from the perspective of lipid metabolism. An integrated analytical approach was employed, combining mass spectrometry imaging (MSI) with liquid chromatography-mass spectrometry (LC-MS), to comprehensively investigate the spatial and temporal dynamics of lipid metabolites during PA exposure. The final lipidomics results combined with RNA sequencing showed that time-dependent changes in metabolite levels after the administration of PAs, involving the pathways of fatty ac

SUBMITTER: Chen Y 

PROVIDER: S-EPMC12856622 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature