Carbon-13 Centerband-Only Detection of EXchange with Dynamic Nuclear Polarization.

ABSTRACT: The magic-angle spinning NMR technique, Centerband-Only Detection of EXchange (CODEX), can be used to determine the oligomerization state of molecules when combined with site-specific labeling. Calibrated with amino acid crystals, the method is successfully applied to proteins, primarily combined with ¹⁹F labeling. The use of ¹³C spins for CODEX-based oligomer determination in proteins is hampered by limited sensitivity of ¹³C spins due to the low gyromagnetic ratio of ¹³C and the presence of natural abundance background spins which contribute to the observed CODEX decay. The use of CODEX is proposed in conjunction with dynamic nuclear polarization (DNP) at low temperature to increase sensitivity. It is necessary to correct for effects of ¹³

SUBMITTER: Cherian Varkey A 

PROVIDER: S-EPMC12856727 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature