Characterization of the Lipidome of Neurons in Mouse Brain Nuclei Using Imaging Mass Spectrometry.

ABSTRACT: Understanding the molecular composition of the brain at cellular level is essential for deciphering the metabolic alterations associated with brain diseases. Furthermore, the different prevalence of some neurological diseases between males and females highlight the importance of incorporating gender factor in such studies. Here, we demonstrate that using imaging mass spectrometry in negative polarity it is possible to isolate and characterize the lipidome of specific neuronal populations in the mouse brain, including the locus coeruleus (LC), mesencephalic neurons and the substantia nigra pars compacta (SNc). Neuronal identity was validated through immunofluorescence on adjacent serial sections. Comparative analysis revealed that each neuronal population presents a distinct and well-define

SUBMITTER: Huergo C 

PROVIDER: S-EPMC12856826 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature