Development and evaluation of quadruplex droplet digital PCR assay for rapid detection of molecular markers associated with macrocyclic lactone resistance and susceptibility in Dirofilaria immitis.

ABSTRACT: Due to climate change and human interventions, there has been an increase in D. immitis infections, underscoring the necessity for monitoring the spread and extent of resistance. In our prior research, we introduced a rapid test utilizing four predominant SNP markers at loci 15709 (SNP1), 30575 (SNP2), 21554 (SNP3), and 9400 (SNP7) linked to ML resistance. Our findings highlighted SNP1 and SNP2 as potent predictive markers, offering suitability for the rapid detection and monitoring of drug resistance. Therefore, we developed a cost-effective test using droplet digital PCR (ddPCR) technology to perform a quadruplex assay to assess alternate allele frequency. Our assay can identify both SNP1 and SNP2 wildtype and mutant targets in a single sample. We tested the performance of our 4-plex ass

SUBMITTER: Kumar S 

PROVIDER: S-EPMC12856862 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature