Integrated Native Mass Spectrometry Imaging of Soluble and Membrane Proteins.

ABSTRACT: Native ambient mass spectrometry enables the analysis of intact protein complexes directly from fresh frozen tissue sections together with visualization of their spatial distribution as part of a mass spectrometry imaging workflow. Native mass spectrometry imaging typically employs nanospray-desorption electrospray ionization (nano-DESI), a liquid junction sampling approach. Imaging of both soluble and membrane proteins has been demonstrated by native nano-DESI but, crucially, imaging of one protein type has always been at the expense of the other, requiring tailored sample preparation and multiple tissue sections. Here, we introduce a new mode of nano-DESI operation that combines soluble and membrane protein analysis into a single experiment, requiring no sample preparation and only a sin

SUBMITTER: Hale OJ 

PROVIDER: S-EPMC12856882 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature