Fluorescence Transduction of Liquid Crystal Ordering Transitions for Biosensing.

ABSTRACT: Liquid crystal (LC) ordering transitions are exquisitely sensitive to molecular interactions at aqueous interfaces and have long served as the basis for optical biosensors. However, the readout of these transitions has almost exclusively relied on polarized-light optical microscopy, which limits quantification and hinders practical deployment. Here, we report a fluorescence-based transduction scheme that converts LC ordering transitions to quantitative optical outputs. Our strategy employs amphiphilic block copolymers bearing aggregation-induced emission (AIE) motifs that undergo dynamic covalent conjugation with IgG antibodies through reversible imine chemistry. In complex LC emulsions, polymer surfactants localize differently depending on droplet LC configuration: accumulation at monopol

SUBMITTER: Vera-Arevalo M 

PROVIDER: S-EPMC12856913 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature