Development, validation, and application of SYBR green-based qPCR assays for detection and quantification of <i>tetA, tetB</i>, and <i>tetO</i> genes in poultry and associated environments.

ABSTRACT:

Introduction

Tetracycline resistance genes (tet genes) are among the most prevalent antimicrobial resistance determinants in poultry, raising concerns about their dissemination across animal, human, and environmental interfaces. This study aimed to develop and validate rapid, sensitive, and cost-effective SYBR Green-based quantitative PCR (qPCR) assays for the detection and quantification of tetA, tetB, and tetO genes in bacterial isolates and complex matrices.

Methods

Following in silico and end-point PCR screening of ten published primer pairs, the most specific combinations were optimized for annealing temperature and primer concentration, and their analytical and diagnostic performances were evaluated.

Results

The assays exhibited effic

SUBMITTER: Piccirillo A 

PROVIDER: S-EPMC12856918 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature