The predictive role of protease-activated receptor (PAR-1) polymorphisms and activated microplatelets on the severity of atherosclerosis - preliminary studies.

ABSTRACT: This study is a comprehensive analysis of PAR-1 - involved in thrombin interaction with platelets (PLT), present on PLT and microparticles (PMP) - to understand its role in diabetic macroangiopathy (DM) and atherosclerosis obliterans (AO). The applied RT-PCR, aggregometry, flow cytometry, a proprietary method for PMP level determination, ELISA, and multidimensional statistical analysis allowed for the determination of: PAR-1 activation levels, its polymorphisms, PLT/PMP aggregation capacity, hemostatic factors, and their interrelationships. In DM, the -506D/D and IVS-14A/A polymorphisms were significantly more frequent, whereas the -506I/D was much more common in AO, suggesting the protective properties of the I allele and its potential significance as a prognostic factor for a milder cour

SUBMITTER: Jakobsche-Policht U 

PROVIDER: S-EPMC12856924 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature