A synergistic dual-mode biosensor for methotrexate detection leveraging FRET and Ag NP-based UV absorption.

ABSTRACT: A novel dual-mode sensing platform (DMSP) integrating fluorescence and ultraviolet (UV) detection was developed for the sensitive and accurate quantification of methotrexate (MTX). This approach combines a FRET-based fluorescent "turn-on" mechanism, employing FAM as the fluorophore and BHQ as the quencher, with a UV absorption mode utilizing the unique surface plasmon resonance properties of silver nanoparticles (Ag NPs). The fluorescence mode enables highly sensitive detection via target-induced structural switching, while the AgNP-based UV channel provides a complementary, concentration-dependent signal at 405 nm, allowing cross-validation and improved reliability. The platform demonstrates a good linear detection range (0.5-20 µM), with detection limits of 0.56 µM and 0.62 µM for

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC12856982 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature