Expression of ultralong complementarity determining region 3 and development of IgM and IgG B cell receptor repertoires in Holstein heifer calves.

ABSTRACT: There are currently knowledge gaps regarding the development of the bovine B cell repertoire especially for cells expressing B cell receptors with heavy chain ultralong complementarity determining region 3 (CDR3). The objective of this study was to assess percentages of B cells with ultralong CDR3s, and immunoglobulin heavy chain gene usage in Holstein calves from birth to 10 mo of age. Blood was collected from 7 heifer calves on the following days of life: 0 (prior to ingestion of colostrum), 2 (after colostrum ingestion), 42, 56, 90, and 285. IgM+ and IgG+ B cells were collected using magnetic-bead separation. RNA was extracted, cDNA was produced, and IgM and IgG sequences were amplified using polymerase chain reactions. Amplicons were sequenced using long-read Oxford Nanopore sequencing

SUBMITTER: Altvater-Hughes TE 

PROVIDER: S-EPMC12857222 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature