Engineering human bone marrow-derived mesenchymal stromal cell aggregates for enhanced extracellular vesicle secretion in a vertical-wheel bioreactor.

ABSTRACT:

Introduction

Human mesenchymal stem/stromal cells (hMSCs) hold significant regenerative potential due to their anti-inflammatory and pro-angiogenic secretome. Three-dimensional (3D) hMSC aggregates secrete extracellular vesicles (EVs) with enhanced immunomodulatory properties compared to 2D cultures. However, the clinical translation of hMSC-EVs remains limited by low production yield. This study investigates scalable EV generation from 3D hMSC aggregates in a novel Vertical-Wheel Bioreactor (VWBR), leveraging shear stress-mediated biochemical cues to enhance EV biogenesis and cargo relevant to nerve regeneration.

Methods

Bone marrow-derived hMSCs were cultured as 3D aggregates in VWBRs and exposed to two different culture media-αMEM/FBS (serum-containing) and DMEM/F12/B27 (

SUBMITTER: Berry D 

PROVIDER: S-EPMC12857299 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature