Engineering <i>Escherichia coli</i> for robust Co-utilization of glucose and xylose enables high-titer succinate production from lignocellulosic hydrolysates.

ABSTRACT: Succinate is a biobased platform chemical with wide applications in food, pharmaceuticals, and biodegradable polymers such as polybutylene succinate. Despite advances in microbial fermentation, cost-effective production remains limited by inefficient utilization of lignocellulosic hydrolysates, where glucose and xylose are the predominant sugars. In this study, we systematically engineered Escherichia coli C600 to enhance succinate biosynthesis from mixed sugars and hydrolysates. Competing by-product pathways were eliminated, the phosphotransferase system was modified to relieve carbon catabolite repression, and the pck gene from Bacillus subtilis was introduced to alleviate the ATP burden in xylose metabolism. To further improve xylose utilization, heterologous oxidat

SUBMITTER: Lv J 

PROVIDER: S-EPMC12857410 | biostudies-literature | 2026 Sep

REPOSITORIES: biostudies-literature