Glycan-binding specificities of anti-ABO(H) antibodies and lectins.

ABSTRACT:

Background

ABO blood type is critically important for blood transfusion and organ transplantation. Blood group reactive glycan-binding proteins are used as reagents in clinical and research laboratories to detect the glycan epitopes that define ABO blood type. The glycan specificities of these clinically used reagents are not well defined.

Methods and materials

We used a glycan microarray to evaluate the specificities of a series of ABO(H) glycan-binding proteins: Ulex europaeus agglutinin I (UEA-I), Helix pomatia agglutinin (HPA), Dolichos biflorus agglutinin (DBA), anti-A antibody, and anti-B antibody.

Results

UEA-I binds many but not all H antigens and binds to some glycans that do not have the H antigen. HPA binds to terminal α-linked N-acetylgalactosamine, as fou

SUBMITTER: Hamm P 

PROVIDER: S-EPMC12857864 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature