Anti-orthopoxvirus drugs inhibit lumpy skin disease virus replication by targeting viral DNA polymerase.

ABSTRACT: Lumpy skin disease (LSD) is an emerging transboundary disease caused by lumpy skin disease virus (LSDV), posing significant threats to global cattle health in the absence of validated drugs. Here, we constructed a recombinant LSDV (rLSDV) expressing both mCherry and luciferase reporters for high-throughput drug screening, and the rLSDV retained virological characteristics phenotypically indistinguishable from the parental strain, with the reporter genes serving as precise and sensitive quantitative indicators for viral replication. Leveraging this platform, we identified six candidates from a library of anti-orthopoxvirus compounds, namely cytarabine (AraC), enrofloxacin (ENR), idoxuridine (IDU), fialuridine (FIAU), ribavirin (RBV), and vidarabine (AraA), demonstrating high antiviral activ

SUBMITTER: Gong Z 

PROVIDER: S-EPMC12858068 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature