Targeting cobra venom cytotoxin: a linear 40-mer ssDNA aptamer-based antivenom confers neutralisation potentials against cobra venom-induced cytotoxicity.

ABSTRACT: Cytotoxin (CTX) is one of the major cobra venom components that contributes to dermonecrosis due to its cytotoxicity. However, current antibody-based antivenoms exert limited neutralisation effects against CTX-induced dermonecrosis. This study focused on discovering aptamer-based antivenom that specifically targets CTX, using repetitive centrifugation-based Systematic Evolution of Ligands by EXponential enrichment (SELEX) selection approach and Illumina amplicon next-generation sequencing. A total of 12 repetitive centrifugation-based selection rounds including a negative selection between rounds 7 and 8 were performed. This was followed by amplicon next-generation sequencing and sequencing bioinformatics workflow to analyse the abundance and persistence of the CTX-binding candidates. Sequ

SUBMITTER: Hiu JJ 

PROVIDER: S-EPMC12858506 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature