Cyclization of γ-hydroxybutyric acid (GHBA) as a strategy to enhance its signal in gas chromatography analysis.

ABSTRACT:

Purpose

The aim of this work is to investigate whether precyclization of γ-hydroxybutyric acid (GABA) allows for increasing its gas chromatography (GC) signal, and if so, is it a more effective way to increase the signal of this compound than its silylation or methylation?

Methods

Gas chromatography-mass spectrometry (GC-MS) and GC with flame ionization detection (GC-FID) response to GHBA before and after silylation, methylation, and cyclization were compared. The impact of injector temperature on GHBA and γ-butyrolactone (GBL) signals was assessed. Fourier transformed infra-red spectroscopy was used to examine the formation of macromolecular derivatives in the injector.

Results

GHBA shows a lower GC signal than GBL due to partial polycondensation into a non-volatile

SUBMITTER: Typek R 

PROVIDER: S-EPMC12858631 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature