A Retrospective Study of the Correlation Between Next-Generation Sequencing and Immunohistochemical Detection of TP53 in Colorectal Cancer.

ABSTRACT: IntroductionThis retrospective study aimed to investigate the correlation between TP53 identified via next-generation sequencing (NGS) and p53 expression in colorectal adenocarcinoma (CRC), as assessed by immunohistochemistry (IHC). Additionally, we characterized p53 IHC staining patterns and sought to determine the optimal threshold for p53 expression as a surrogate for TP53 mutation status.MethodsIn this retrospective cohort analysis, we included 294 archived surgically resected CRC specimens from patients who did not receive preoperative chemotherapy were analyzed. All data were collected from pathology database and electronic medical records. TP53 mutations were identified using NGS, and p53 expression was evaluated by IHC. The correlation between mutation status a

SUBMITTER: Wu J 

PROVIDER: S-EPMC12858738 | biostudies-literature | 2026 Jan-Dec

REPOSITORIES: biostudies-literature