Transgelin defines pro-tumorigenic cancer-associated fibroblasts in pancreatic cancer.

ABSTRACT:

Introduction

Pancreatic ductal adenocarcinoma (PDAC) is characterised by a pronounced desmoplastic reaction, predominantly composed of cancer-associated fibroblasts (CAFs), including myofibroblastic CAFs (myCAFs).

Methods

We performed a single-cell assay for transposase-accessible chromatin with high-throughput sequencing (scATAC-seq) and single-cell RNA sequencing (scRNA-seq) on pancreas tissues from KPC mice (LSL-Kras^G12D/+; LSL-Trp53^R172H/+; Pdx-1-Cre) to characterise myCAF heterogeneity. A transgelin (Tagln) knockout orthotopic mouse model was used to determine the functional role of Tagln.

Results

Epigenetic profiling uncovered heterogeneity within the myCAF population, revealing distinct subclusters characterised by specific transcription f

SUBMITTER: Wang X 

PROVIDER: S-EPMC12858813 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature