Salmonella typhimurium co-expressing cytolysin A and hyaluronidase suppresses tumor growth and metastasis.

ABSTRACT: Recently, various attenuated bacteria have been studied as cancer therapies due to their unique characteristics, which include tumor-targeting bioactivity and immunogenicity. Previously, we reported a Salmonella typhimurium strain, CNC018, which is attenuated by 10⁵-10⁶-fold compared with the wild-type strain but retains tumor-targeting specificity. However, although these bacteria suppress tumors at the early stage in mice, the tumors often regrow at later stages. Therefore, to increase antitumor efficacy, we used a doxycycline-inducible system to engineer this strain (CNC018pCH) to secrete both cytolysin A (ClyA) and hyaluronidase (HysA), a pore-forming toxin that kills tumor cells and an enzyme that disrupts the tumor microenvironment, respectively. Local secretion of ClyA from CNC018pC

SUBMITTER: Nguyen KV 

PROVIDER: S-EPMC12859083 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature