Loss of Cathepsin K Impairs Collagen Biogenesis and Enhances Actin Polymerization in Trabecular Meshwork.

ABSTRACT:

Purpose

Compromise in trabecular meshwork (TM) function due to extracellular matrix (ECM) accumulation contributes to increased intraocular pressure (IOP) in primary open-angle glaucoma (POAG). Our previous study demonstrated the role of cathepsin K (CTSK), a potent collagenase, on ECM homeostasis, actin bundling in TM, and IOP regulation. This study was designed to understand the response of TM cells to the loss of CTSK function.

Methods

Normal primary human TM (HTM) cells transfected with either small interfering RNA (siRNA) against CTSK (siCTSK) or scrambled siRNA (siScr) as a control were screened using mass spectrometry-based quantitative proteomics for changes in protein levels. Immunofluorescence imaging was used to identify changes in the distribution of differential

SUBMITTER: Soundararajan A 

PROVIDER: S-EPMC12859711 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature