Monoclonal antibody preparation and linear epitope identification for the ASFV A151R protein.

ABSTRACT: African Swine fever (ASF) is an acute, hemorrhagic infectious disease caused by the African swine fever virus (ASFV), posing a major threat to the global pig farming industry. ASFV is a complex DNA virus. Its non-structural protein, A151R, is a thioredoxin crucial for the replication and morphogenesis of ASFV. In this study, a monoclonal antibody (mAb) 3E5D6 against pA151R (A151R Protein) was prepared using cell fusion technology to gain deeper insight into the function of A151R during ASFV infection. Immunolabeling of pA151R with 3E5D6 mAb revealed its involvement in viral factory formation and its characteristic early expression during infection. However, the antibody exhibited no detectable neutralizing activity. Subsequently, the antigenic epitope ⁸⁴KIWSGEPR⁹¹ of

SUBMITTER: Liu Z 

PROVIDER: S-EPMC12859835 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature