Genomic and functional characterization of a novel lytic phage vB-AbaM-fThrA with anti-biofilm activity against CR-MDR Acinetobacter baumannii.

ABSTRACT:

Background

Multidrug-resistant Acinetobacter baumannii causes increasingly fat5al infections in humans. Phage therapy is considered as a promising approach to treat the infections.

Methods

Here we present the isolation and characterization of a new lytic A. baumannii specific phage vB-AbaM-fThrA (fThrA for short) originating from hospital wastewater.

Results

Phage fThrA has a myovirus morphology with a relatively broad host range. The phage forms large and transparent plaques with a halo and showed high lysis power in in-vitro laboratory studies. Based on the genome sequence analysis phage fThrA is a new member of the Obolenskvirus genus with about 75.6% genomic similarity to its closest relative. The phage, tolerated well pH range from 4 to 10, and heating at 50 °C,

SUBMITTER: Arazi P 

PROVIDER: S-EPMC12859838 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature