DACT1 inhibits cuproptosis and promotes cell malignancy via activation of PI3K/AKT signaling in laryngeal squamous cell carcinoma.

ABSTRACT:

Background

Laryngeal cancer has one of the highest mortality rates of all head and neck cancers. DACT1 is a cuproptosis-related gene in laryngeal cancer and serves as a risk factor for patient prognosis. This study aimed to investigate the effects of DACT1 on the malignant behavior and cuproptosis of laryngeal squamous cell carcinoma (LSCC) cells.

Methods

DACT1 expression in LSCC cells was measured using RT-qPCR and western blotting. To establish cuproptosis cell model, TU212 and TU686 cells were incubated with elesclomol (20 nM) and CuCl₂ (20 nM) for 24 h. Transfection of shRNA or pcDNA3.1 vectors was performed to interfere DACT1 expression. LY294002 (PI3K inhibitor) and 740Y-P (PI3K agonist) were used to silence and overexpress PI3K signaling in LSCC cells, resp

SUBMITTER: Guo Y 

PROVIDER: S-EPMC12859876 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature