Different plasma exosome isolation methods generated distinct microRNA and protein profiles in healthy controls and patients with advanced prostate and lung cancer.

ABSTRACT:

Background

Exosomes, extracellular vesicles pivotal in cancer intercellular communication, encapsulate biomolecules with potential as diagnostic and prognostic biomarkers. Efficient isolation is essential for accurate molecular profiling. This study compares three exosome isolation methods-size exclusion chromatography (SEC), lectin-binding, and TIM4-binding-for proteomic and miRNA analysis of plasma exosomes in cancer.

Methods

Plasma exosomes from patients with non-small cell lung cancer (NSCLC, N = 22), castration-resistant prostate cancer (CRPC, N = 7), and healthy controls (N = 15) were analyzed. Liquid chromatography-tandem mass spectrometry profiled exosomal proteins, and small RNA sequencing identified miRNAs.

Results

SEC, lectin-binding, and TIM4-binding metho

SUBMITTER: Avasthi KK 

PROVIDER: S-EPMC12859987 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature