Cryogenic microcarrier-assisted stem cell storage (Cryo-MASCS): a preservation method enabling scalable culture of human mesenchymal stem cells.

ABSTRACT: Microcarrier (µC) suspension systems enable closed, high-density manufacturing of human mesenchymal stem cells (MSCs), but current workflows remain labor-intensive because they require post-thaw cryoprotectant removal and static culture steps to allow cell attachment. To address these bottlenecks, we developed a cryogenic microcarrier-assisted stem cell storage (Cryo-MASCS) workflow that integrates cell attachment, cryopreservation, and thawing directly on surface-engineered µCs. MSCs were pre-seeded onto µCs coated with stacked heparan sulfate-collagen bilayers, cryopreserved on-carrier in either conventional dimethyl sulfoxide (DMSO)-supplemented medium or a DMSO-free, serum-free (SF) minimally supplemented medium, and then thawed and returned directly to suspension culture without inter

SUBMITTER: Rivera-Crespo C 

PROVIDER: S-EPMC12859993 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature