Protein tyrosine kinase Src suppresses hepatitis C virus particle release through regulation of Ndrg1.

ABSTRACT: Tyrosine kinases are known to regulate multiple stages of the hepatitis C virus (HCV) life cycle. We previously demonstrated that Abl kinase facilitates viral particle assembly; however, the roles of other tyrosine kinases remain largely undefined. In this study, we evaluated the antiviral potential of tyrosine kinase inhibitors (TKIs) and investigated the associated host regulatory mechanisms. Screening a panel of clinically approved TKIs in HCV-infected Huh-7.5 cells revealed that Bosutinib, a dual inhibitor of Abl and Src kinases, significantly reduced extracellular viral titers. Unexpectedly, CRISPR/Cas9-mediated knockout of Src kinase had no effect on viral replication, protein synthesis, or assembly but markedly enhanced the release of infectious particles. We further identified N-my

SUBMITTER: Fu L 

PROVIDER: S-EPMC12860343 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature