Global discovery of RNA modifications and functional analysis of m⁵C methylome in cyanobacteria.

ABSTRACT: RNA modifications have been found in all domains of life and play regulatory roles in diverse biological processes. However, their distribution, function, and regulation in cyanobacteria remain unexplored. Here, we have employed a quantitative RNA profiling strategy based on mass spectrometry analysis to identify 21 different RNA modifications in the model cyanobacterium Synechocystis sp. PCC 6803 (Synechocystis). Mass spectrometry analyses reveal a dynamic pattern of these RNA modifications under different culture conditions. We subsequently perform transcriptome-wide 5-methylcytosine (m⁵C) profiling in Synechocystis by using bisulfite sequencing. In total, we identify 824 high-confidence m⁵C sites in 382 mRNAs, with the majority of m⁵C-modified genes part

SUBMITTER: Cao G 

PROVIDER: S-EPMC12860351 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature