Single-Chain Anti-IL-1β Antibody Carried by Outer Membrane Vesicles of Bacteroides fragilis Alleviates Tubular Inflammation in Chronic Kidney Disease.

ABSTRACT: Microinflammation is a key driver of chronic kidney disease (CKD) progression, with interleukin-1β (IL-1β) playing a pivotal role. However, current anti-IL-1β antibody therapies face critical limitations, such as systemic side effects and substantial production costs, which hinder their therapeutic efficacy and clinical translation for CKD intervention. To address this, we developed Bacteroides fragilis-derived outer membrane vesicles (OMVs) encapsulating anti-IL-1β single-chain variable fragment (scFv) and conjugated with kidney-targeting peptides (KKEEE)₃K. Engineered OMV-(KKEEE)₃K-scFv's safety was evaluated in vitro (24, 48 h) and in vivo (2 months). Its anti-inflammatory efficacy was assessed in a high glucose-induced model in vitro, and in various kidney disease

SUBMITTER: Li L 

PROVIDER: S-EPMC12860400 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature