Targeted identification of new phaterpenes and elucidation of the relevant biosynthetic pathway in <i>Streptomyces phaeochromogenes</i> OSK-123.

ABSTRACT: Sesquiterpenes have highly diverse chemical structures and biological activities, resulting in considerable interest in terms of their application and exploration of new analogs. In this study, we identified a new cryptic bacterial terpenoid biosynthetic gene cluster (i.e., pha) in the genome of the soil microorganism Streptomyces phaeochromogenes OSK-123 (strain OSK-123). According to online BiG-FAM and sequence similarity network analyses, pha was revealed to span an approximately 18 kb DNA region with 14 open reading frames, which likely include sequences encoding enzymes catalyzing the production of new sesquiterpenes. To facilitate the identification of target compounds, strong constitutive promoters were incorporated into pha. Additionally, we conducted a

SUBMITTER: Fan X 

PROVIDER: S-EPMC12860625 | biostudies-literature | 2026 Sep

REPOSITORIES: biostudies-literature