Engineering <i>Escherichia coli</i> for polyethylene terephthalate powder biodegradation via recoding of an outer membrane protein.

ABSTRACT: Deep computationally guided protein design enabled the introduction of a Ser-His-Asp catalytic triad that supports polyethylene terephthalate (PET) hydrolysis within an inner membrane Escherichia coli protein. This allows the engineering, through gene editing, of a strain capable of degrading PET particles smaller than 4.5 nm. We extended this approach to PET powder (<300 μm) using a computational workflow that builds geometrically pre-organized catalytic triads while preserving substrate binding in extracellular or surface-exposed membrane proteins. Four additional proteins were reprogrammed to degrade PET. Replacement of the outer membrane protein OmpA with a PETase-active variant carrying a surface-exposed artificial triad enabled an engineered strain to release 157 ± 2 μM hydrol

SUBMITTER: Gimenez-Dejoz J 

PROVIDER: S-EPMC12860643 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature