Efferocytosis-associated transcriptomic patterns characterize prognosis and immune landscape in osteosarcoma.

ABSTRACT:

Background

Osteosarcoma (OS) is the most common primary malignant bone tumor in adolescents, characterized by high heterogeneity and poor prognosis. Efferocytosis, the clearance of apoptotic cells, has been implicated in tumor progression and immune evasion, but its role in OS remains unclear.

Methods

We integrated TARGET-OS as a training cohort with three GEO datasets for validation. Efferocytosis pathways were quantified by ssGSEA, and WGCNA was applied to identify associated gene modules. Candidate genes were screened using univariate Cox regression, and a prognostic signature was developed with machine learning models and validated across cohorts. Functional enrichment, immune infiltration, and immunotherapy prediction analyses were performed. scRNA-seq from six OS patie

SUBMITTER: Song X 

PROVIDER: S-EPMC12860682 | biostudies-literature | 2026 Apr

REPOSITORIES: biostudies-literature