Actn4 Links Inactive Integrin α5 With Actin in Zebrafish Somites.

ABSTRACT: Integrins are key plasma membrane proteins that mediate cell-ECM adhesion and communication, and they rely on a conformational change for their activation and bidirectional signaling. However, there are few in vivo studies of integrin activation. Here, we identify Integrin α5 (Itgα5)-associated proteins in the physiological setting of zebrafish somite morphogenesis. Using label-free mass spectrometry, we compared Itgα5-associated proteins in different integrin activation states. As expected, we found active Itgα5 enriched extracellular matrix (ECM) proteins. Surprisingly, inactive Itgα5 incapable of binding ligand recruits actin cytoskeletal proteins as efficiently as the active integrin. We validated Itgα5's linking to actin adaptors using Parallel Reaction Monitoring (PRM). We then focus

SUBMITTER: Sun G 

PROVIDER: S-EPMC12860946 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature