The construction of recombinant DNA vaccine pVAX-ROP27-IL-2-IFN-γ and its immune enhancement effect against Eimeria tenella.

ABSTRACT: Eimeria tenella (E. tenella) is a highly pathogenic and widely distributed species responsible for significant economic losses in the global poultry industry. The rhoptry protein 27 (EtROP27) DNA vaccine of E. tenella provides certain immune protection against E. tenella infection. Notably, vaccination triggers a sharp increase in the expression of chicken-derived cytokines chIL-2 and chIFN-γ. This study combines chIL-2 and chIFN-γ with the EtROP27 DNA vaccine to investigate the immunomodulatory effects. Specific-pathogen-free (SPF) chickens were immunized intramuscularly, and successful in vivo expression was confirmed by Reverse Transcription Polymerase Chain Reaction and Western blot. Immunoprotective assays, histopathology, enzyme-linked immunosorbent assay (ELISA), quantitative polyme

SUBMITTER: Niu Z 

PROVIDER: S-EPMC12861031 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature