High glucose upregulates BMAL1 in chondrocytes from macroscopically normal and osteoarthritic human cartilage but this only results in increased collagen synthesis in chondrocytes from macroscopically normal cartilage regions.

ABSTRACT:

Objective

Osteoarthritic (OA) chondrocytes exhibit limited capacity for new collagen synthesis. High glucose promotes collagen I and III production by fibroblasts. The purpose of this study was to determine if it also promotes collagen II (COL2) synthesis by chondrocytes.

Design

Chondrocytes isolated from osteoarthritic (OA) and macroscopically normal (MN) cartilage from patients with knee OA were maintained in 1 % oxygen in 4 mM or 10 mM glucose for up to 24 h.

Results

Secreted COL2 levels were higher in MN but not OA chondrocytes maintained in 10 mM versus 4 mM glucose with increased COL2 production specifically evident in the first 6 h of high glucose exposure. COL2A1 and proly-4-hydroxylase α2 (P4HA2) RNA and protein were upregulated by high glucose in MN a

SUBMITTER: Jain L 

PROVIDER: S-EPMC12861034 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature