Effects of graded phosphate deficiency and vitamin D intervention on growth, bone metabolism, and mineralization in a rat model of neonatal-onset metabolic bone disease.

ABSTRACT: To investigate the dose-dependent effects of neonatal-onset phosphate deficiency on bone growth and mineralization and assess whether supraphysiological vitamin D₃ or calcitriol can rescue skeletal defects. Newborn Sprague-Dawley rats were randomly assigned to 7 diets: phosphate-free (0P), low phosphate (1/2P), normal phosphate (NP), calcium-free (0Ca), phosphate/vitamin D-free (0P/D), and 0P/D supplemented with either supraphysiological vitamin D₃ (0P/D + D₃) or calcitriol (0P/D + calcitriol). Longitudinal radiographic assessments were performed before euthanasia at 6-8 wk. Serum analyses measured phosphate (sP), calcium (sCa), phosphotropic hormones, and bone turnover markers. Tibial growth plates were examined by H&E staining, micro-CT, and histomorphome

SUBMITTER: Wu S 

PROVIDER: S-EPMC12861084 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature