Comparative Multi-Omics Analysis of the Iridocorneal Angle Identifies an Immune-Fibrotic Profile in the DBA/2J Glaucoma Mouse Model.

ABSTRACT: We present the first integrated transcriptomic and proteomic profiling of the iridocorneal region in the spontaneous murine glaucoma model DBA/2J and DBA/2J-Gpnmb⁺/Sj controls to define molecular changes associated with ocular hypertension and glaucoma. Using RNA sequencing and label-free quantitative proteomics, we identified over 20,000 transcripts and 8500 proteins, creating a comprehensive molecular atlas of glaucoma-related alterations in DBA/2J mice. Principal component and differential expression analyses revealed distinct genotype-specific molecular signatures. In DBA/2J mice, upregulated genes were enriched in pathways related to extracellular matrix remodeling, collagen organization, TGF-β signaling, and inflammation. Proteomic data confirmed increased levels of comple

SUBMITTER: Shim MS 

PROVIDER: S-EPMC12861312 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature