Expression changes of human Schlemm's canal endothelial cells in response to cyclic mechanical stretch.

ABSTRACT: The exact mechanisms whereby Schlemm's canal endothelial cells (SCECs) regulate intraocular pressure (IOP) homeostasis are not fully understood. We investigated expression changes in messenger RNAs (mRNAs) and long non-coding RNAs (lncRNAs) in primary human SCECs in response to cyclic mechanical stretch (CMS), a known component of both physiologic and pathologic IOP fluctuations. Primary SCECs from healthy human donors (n = 3) were subjected to 15% CMS (1 cycle/sec) for 24-h. Stranded total RNA sequencing and Partek Flow analysis were performed to identify differentially expressed mRNAs and lncRNAs in stretched SCECs. Gene ontology and pathway analyses were performed using WebGestalt and Ingenuity Pathway Analysis. Differential expression of four genes (DHCR7, PMEPA1, SCD, and FIBIN) was v

SUBMITTER: Heo Y 

PROVIDER: S-EPMC12861585 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature