Improvement of EPO Transgene Detection From Polymeric Dried Blood Spots for Antidoping Application.

ABSTRACT: For the past couple of years, black market products have appeared and were confirmed to contain genetic products coding for human erythropoietin (EPO). While being prohibited by the World Anti-Doping Agency (WADA), they could be used to produce endogenously more EPO hormone and hence increase performance. In a previous work, we demonstrated the potential of 20-μL dried blood spots (DBS) to detect the presence of EPO transgene in human blood down to 250 copies (12,500 copies/mL), despite lower sensitivity (30-fold) than in 1-mL fresh blood. As the use of DBS as a collection matrix for antidoping is going to expand in the near future, our aim was to develop and validate a new protocol to improve the sensitivity of gene doping detection from DBS. Three DBS devices were evaluated: polymeric Ta

SUBMITTER: Marchand A 

PROVIDER: S-EPMC12861599 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature