Repeated proliferative events ameliorate age-associated accumulation of DNA damage in HSPCs.

ABSTRACT: Upon aging, hematopoietic stem cells show accumulation of DNA damage that has been causally linked with their functional decline, with debatable role of proliferative events. In this study, we sought to enquire the effect of increased proliferation rate in hematopoietic stem and progenitor cells (HSPCs) on hematopoietic aging. Multiple rounds of blood withdrawals were performed during adult life to maintain a higher proliferation rate in HSPC population in mice. Our experiments showed little effect of increased proliferation on age-associated functional decline in the hematopoietic system. However, we noted a decrease in the double-strand breaks accumulated with age after the serial bleeding regimen. Analysis of scRNA-Seq data from mouse and human HSPCs showed enrichment of DNA damage resp

SUBMITTER: Mehatre SH 

PROVIDER: S-EPMC12861689 | biostudies-literature | 2026 Apr

REPOSITORIES: biostudies-literature