Temporal dynamics of macrophage transcriptional profiles during zebrafish wound healing.

ABSTRACT:

Introduction

Macrophages participate in wound healing by contributing to host defense and by orchestrating inflammation and repair. They adapt dynamically to the wound microenvironment by adopting diverse polarization states, which are determinant of wound outcome, influencing whether healing is successful or becomes chronic. The zebrafish embryo, widely used for live imaging of immune responses, is a powerful model to study macrophage behavior after injury. However, the transcriptional landscape of polarized macrophages in this model during wound healing remains insufficiently characterized.

Methods

Here, we employed bulk RNA sequencing on FACS-sorted zebrafish macrophages at different times following tail fin wounding in larvae, a robust model for studying sterile inflamma

SUBMITTER: Begon-Pescia C 

PROVIDER: S-EPMC12861901 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature