Hydrogen-Mediated Activation of the Nrf2/HO-1 Signaling Pathway Improves Cognitive Impairment in Sleep-Deprived Mice.

ABSTRACT:

Aims

This study investigated the effects of hydrogen (H₂) on cognitive impairment in sleep-deprived mice mediated by the nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling pathway.

Methods

A chronic sleep deprivation (SD) model was established using the modified multiple platform method, with 18 h of deprivation daily for 28 consecutive days. Cognitive function was evaluated using the Morris water maze and novel object recognition (NOR) test. Histopathological and biochemical analyses, including hematoxylin and eosin staining, Nissl staining, immunohistochemistry, and enzyme-linked immunosorbent assay, were performed to assess oxidative stress and inflammation-related markers.

Results

The results demonstrated that H_2</

SUBMITTER: Xiao Q 

PROVIDER: S-EPMC12862102 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature