Chrysin Exhibits Bone-Protective Effects Through Osteoclastogenesis Inhibition: In Vitro and In Vivo Evaluation in RAW 264.7 Murine Macrophages and Sprague-Dawley Rats.

ABSTRACT: Bone is a metabolically active tissue that is constantly being reformed and resorbed by osteoblasts and osteoclasts. Abnormal increases in osteoclast activity can lead to bone deterioration. This study investigated the potential beneficial effects of chrysin on osteoclast formation in RAW 264.7 murine macrophages and bone health in Sprague-Dawley rats. Tartrate-resistant acid phosphatase (TRAP) staining was conducted to determine the effect of chrysin on osteoclast differentiation. Quantitative polymerase-chain reaction, western blotting, and immunofluorescence were conducted to determine the molecular mechanism of chrysin in osteoclasts. Sprague-Dawley rats were fed a diet of 50 mg/kg chrysin from postnatal Day 7 until 22. On Day 130, the rats were euthanized, and their tibiae were extrac

SUBMITTER: Mason C 

PROVIDER: S-EPMC12862122 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature