Optimization and comparison of genomic DNA extraction from whole blood collected in PAXgene blood RNA tube using automated platforms.

ABSTRACT:

Objectives

With the development of genomic technologies, the isolation of genomic DNA (gDNA) from clinical samples has become more important for both clinical diagnostics and research studies. Blood samples collected in PAXgene Blood RNA Tubes, which are typically used for RNA extraction, can be used for gDNA extraction, particularly in clinical studies when only such blood samples are available.

Methods

We optimized the pre-treatment of blood samples collected in PAXgene Blood RNA Tubes. For the first time, three magnetic bead-based automated platforms (QIAsymphony SP, Maxwell RSC and KingFisher Apex) were compared for gDNA extraction from these blood samples. Additionally, the effects of storage at 4 °C or freeze-thaw cycles of the blood samples on gDNA yield were investig

SUBMITTER: You J 

PROVIDER: S-EPMC12862341 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature