Systematic evaluation of long- and short-read RNA-seq for human peripheral blood.

ABSTRACT: RNA sequencing (RNA-seq) technologies enable comprehensive transcriptomic profiling, yet systematic comparisons using identical biological samples remain limited. Here, we performed a multi-faceted comparison of long-read (PacBio) and short-read (Illumina) RNA-seq using the same RNA from peripheral blood cells of four healthy donors. Unlike prior studies that aggregate datasets from different sources, this study evaluates platform-dependent performance across gene expression, transcript variants, fusion genes, primary microRNAs (pri-miRNAs), and immune receptor complementarity-determining region 3 (CDR3) regions using widely available software, highlighting both reproducibility and accessibility. Long-read sequencing outperformed short-read sequencing in detecting complex alternative splic

SUBMITTER: Iwabuchi S 

PROVIDER: S-EPMC12862385 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature