Hyptolide Promotes Breast Cancer Stem Cells Apoptosis and S-phase Cell Cycle Arrest.

ABSTRACT:

Objective

Breast cancer stem cells (BCSCs) develop apoptosis resistance by expressing pro and antiapoptotic proteins. The induction of apoptosis is an important mechanism of action for many anticancer agents. However, recently chemotherapy-induced chemoresistance and increased relapse phenomenon due to BCSCs population-targeted therapy failure. Therefore, treatment using a new compound was supposed to inhibit apoptosis resistance and increase the possibility of cancer apoptosis in the BCSCs population. This study aimed to investigate the effects of the hyptolide, isolate a compound from Hyptis pectinata on apoptosis induction in BCSCs.

Methods

The cytotoxic activity was analyzed using MTT assay. Annexin V-Propidium Iodide measured apoptosis under flow cytometry. Cell cycle a

SUBMITTER: Suzery M 

PROVIDER: S-EPMC12862401 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature